ABSTRACT
Nerve growth factor (NGF) is known to regulate both cancer cell survival and death signaling, depending on the cellular circumstances, in various cell types. In this study, we showed that NGF strongly upregulated the protein level of tropomyosin-related kinase A (TrkA) in TrkA-inducible SK-N-MC cancer cells, resulting in increases in various TrkA-dependent cellular processes, including the phosphorylation of c-Jun N-terminal kinase (JNK) and caspase-8 cleavage. In addition, NGF enhanced TrkA-induced morphological changes and cell death, and this effect was significantly suppressed by the JNK inhibitor SP600125, but not by the phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin. To investigate novel targets associated with the enhancement of TrkA-induced SK-N-MC cell death caused by NGF, we performed Coomassie Brilliant Blue staining and two-dimensional (2D) proteomic analysis in TrkA-inducible SK-N-MC cells. We identified 31 protein spots that were either greatly upregulated or downregulated by TrkA during NGF treatment using matrix-associated laser desorption/ionization time of flight/time of flight mass spectrometry, and we analyzed the effects of SP600125 and wortmannin on the spots. Interestingly, 11 protein spots, including heterogeneous nuclear ribonucleoprotein K (hnRNP K), lamin B1 and TAR DNA-binding protein (TDP43), were significantly influenced by SP600125, but not by wortmannin. Moreover, the NGF/TrkA-dependent inhibition of cell viability was significantly enhanced by knockdown of hnRNP K using small interfering RNA, demonstrating that hnRNP K is a novel target associated with the regulation of TrkA-dependent SK-N-MC cancer cell death enhanced by NGF.
Subject(s)
Caspase 8 , Cell Death , Cell Survival , Heterogeneous-Nuclear Ribonucleoprotein K , JNK Mitogen-Activated Protein Kinases , Mass Spectrometry , Nerve Growth Factor , Phosphatidylinositol 3-Kinase , Phosphorylation , Phosphotransferases , RNA, Small InterferingABSTRACT
Tropomyosin-related kinase A (TrkA) plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types. Here we show that TrkA overexpression by the Tet-On system mimics NGF-mediated activation pathways in the absence of nerve growth factor (NGF) stimulation in U2OS cells. In addition, p53 upregulation upon DNA damage was inhibited by TrkA, and p21 was upregulated by TrkA in a p53-independent manner. TrkA overexpression caused cell death by interrupting cell cycle progression, and TrkA-induced cell death was diminished in the presence of its specific inhibitor GW441756. Interestingly, TrkA-mediated cell death was strongly related to gammaH2AX production and poly (ADP-ribose) polymerase cleavage in the absence of DNA damage inducer. In this study, we also reveal thatgammagammaH2AX production by TrkA is blocked by TrkA kinase inhibitors K-252a and GW441756, and it is also significantly inhibited by JNK inhibitor SP600125. Moreover, reduction of cell viability by TrkA was strongly suppressed by SP600125 treatment, suggesting a critical role of JNK in TrkA-induced cell death. We also found that gammaH2AX and TrkA were colocalized in cytosol in the absence of DNA damage, and the nuclear localization of gammaH2AX induced by DNA damage was partly altered to cytosol by TrkA overexpression. Our results suggest that the abnormal cytosolic accumulation of gammaH2AX is implicated in TrkA-induced cell death in the absence of DNA damage.
Subject(s)
Humans , Anthracenes/pharmacology , Apoptosis/drug effects , Carbazoles/pharmacology , Cell Cycle/drug effects , Cell Line, Tumor , Cyclin-Dependent Kinase Inhibitor p21/biosynthesis , Cytosol/drug effects , DNA Damage/drug effects , Doxorubicin/pharmacology , Histones/metabolism , Indole Alkaloids/pharmacology , MAP Kinase Kinase 4/antagonists & inhibitors , Nerve Growth Factor/antagonists & inhibitors , Phosphorylation/drug effects , Protein Binding , Protein Transport/drug effects , Receptor, trkA/antagonists & inhibitors , Signal Transduction , TransfectionABSTRACT
Rheumatoid arthritis (RA) is a chronic inflammatiory disease that mainly destroys cartilages or bones at the joints. This inflammatory disorder is initiated by self-attack using own immune system, but the detail of pathological mechanism is unclear. Features of autoantigens leading to autoimmune disease are also under veil although several candidates including type II collagen have been suggested to play a role in pathogenesis. In this report, we tried to identify proteins responding to antibodies purified from RA patients and screen proteins up-regulated or down-regulated in RA using proteomic approach. Fibronectin, semaphorin 7A precursor, growth factor binding protein 7 (GRB7), and immunoglobulin mu chain were specifically associated with antibodies isolated from RA synovial fluids. In addition, some metabolic proteins such as adipocyte fatty acid binding protein, galectin-1 and apolipoprotein A1 precursor were overexpressed in RA synovium. Also, expression of peroxiredoxin 2 was up-regulated in RA. On the contrary, expression of vimentin was severely suppressed in RA synoviocytes. Such findings might give some insights into understanding of pathological mechanism in RA.
Subject(s)
Middle Aged , Male , Humans , Female , Aged , Adult , Synovial Fluid/metabolism , Sepharose/chemistry , Proteomics/methods , Inflammation , Gene Expression Regulation , Collagen Type II/biosynthesis , Autoantigens/metabolism , Arthritis, Rheumatoid/metabolismABSTRACT
Renal cell carcinoma (RCC) is one of the most malignant tumors in urology, and due to its insidious onset patients frequently have advanced disease at the time of clinical presentation. Thus, early detection is crucial in management of RCC. To identify tumor specific proteins of RCC, we employed proteomic analysis. We prepared proteins from conventional RCC and the corresponding normal kidney tissues from seven patients with conventional RCC. The expression of proteins was determined by silver stain after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The overall protein expression patterns in the RCC and the normal kidney tissues were quite similar except some areas. Of 66 differentially expressed protein spots (p<0.05 by Student t-test), 8 different proteins from 11 spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p<0.05); aminoacylase-1, enoyl-CoA hydratase, aldehyde reductase, tropomyosin alpha-4 chain, agmatinase and ketohexokinase. Two proteins, vimentin and alpha-1 antitrypsin precursor, were dominantly expressed in RCC (p<0.05).
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Aldehyde Reductase/analysis , Amidohydrolases/analysis , Carcinoma, Renal Cell/metabolism , Comparative Study , Electrophoresis, Gel, Two-Dimensional , Enoyl-CoA Hydratase/analysis , Fructokinases/analysis , Kidney Neoplasms/metabolism , Proteome/analysis , Proteomics/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Tropomyosin/analysis , Ureohydrolases/analysis , Vimentin/analysis , alpha 1-Antitrypsin/analysisABSTRACT
CPTP1 is a nontransmembrane chicken protein tyrosine phosphatase having 92% sequence homology to the corresponding 321 amino acids of human protein tyrosine phosphatase 1B (HPTP1B). Using anti-CPTP1 antibody, we identified CPTP1-like rat PTP1 of 51 kappa Da in Rat-1 and v-src-transformed Rat-1 fibroblasts. Here we show that CPTP1-like rat PTP1 binds to p60v-src in vivo and CPTP1 also can associate with p60v-src in cell lysate of v-src- transformed Rat-1 fibroblasts. Interaction between HPTP1B-type PTPs, CPTP1-like rat PTP1 and CPTP1, and p60v-src was reduced by vanadate treatment for 13 h due to down regulation of the protein level of p60v-src in vivo. Interestingly, CPTP1-like rat PTP1 was coimmunoprecipitated with a 70-kappa Da protein which has a possibility to be tyrosine- phosphorylated by p60v-src in v-src-transformed Rat- 1 fibroblasts. These results suggest that HPTP1B- type PTPs may play an important role in p60src dependent signal pathway in eucaryotic cells.
Subject(s)
Animals , Female , Rabbits , Rats , Blotting, Western , Cell Line, Transformed , Chickens , Fibroblasts , Oncogene Protein pp60(v-src)/metabolism , Phosphoprotein Phosphatases/genetics , Precipitin Tests , Protein Binding , Protein Tyrosine Phosphatases/genetics , Recombinant Fusion Proteins/geneticsABSTRACT
Protein tyrosine phosphorylation and dephosphorylation are important in the regulation of cell proliferation and signaling cascade. In order to examine whether phosphatase activity of CPTP1 and HPTP1B, typical nontransmembrane protein tyrosine phosphatase, could be controlled by phosphorylation, affinity-purified PTPs were phosphorylated by CKII and p56lck in vitro. Phosphoamino acid analysis revealed that CPTP1 was phosphorylated on both serine and threonine residues by CKII, and tyrosine residue by p56lck. Phosphatase activity of CPTP1 was gradually increased by three-fold concomitant with phosporylation by CKII. Phosphorylation of HPTP1B by CKII resulted in quick two-fold enhancement of its phosphatase activity within 5 min of incubation and remained in that state. In the presence of CKII inhibitor, heparin or poly(Glu.Tyr), both phosphorylation and enhancement of phosphatase activity of CPTP1 and HPTP1B were mostly blocked. p56lck catalyzed tyrosine phosphorylation of CPTP1 and HPTP1B was only observed by inhibiting the intrinsic tyrosine phosphatase activity. Taken together, these results indicate that CPTP1 or HPTP1B possesses a capability to regulate its phosphatase activity through phosphorylation processes and may participate in the cellular signal cascades.
Subject(s)
Humans , Adenosine Triphosphate/metabolism , Animals , Chickens , Dose-Response Relationship, Drug , Heparin/pharmacology , Hydrogen Peroxide/pharmacology , Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/metabolism , Peptides/pharmacology , Phosphorus Radioisotopes , Phosphorylation/drug effects , Protein Serine-Threonine Kinases/metabolism , Protein Tyrosine Phosphatases/metabolism , Tyrosine/metabolism , Vanadates/pharmacologyABSTRACT
The increase of serum IgE in atopic dermatitis is well known. And atopic patients are frequently complicated by viral infection, such as molluscum contagiosum. So, we studied the serum IgE level in patients of atopic dermatitis and atopic dermatitis with molluscum eontagiosum to evaluate the relationship between molluscum contagiosum and IgE level in patients with atopic dermatitis. The results were summarized as follows : 1. Serum IgE level in patients of atopic dermatitis was 168.8+117.0 IU/ml, which was higher than that of control group (87.6+/-40.0 IU/ml) (p0.05).
Subject(s)
Humans , Dermatitis, Atopic , Immunoglobulin E , Molluscum ContagiosumABSTRACT
We report a case of 21-year-old patient who has had dome-shaped nodules 1 cm in diameter on the right shoulder for one year. Histopathologic findings showed central dilated cystic hair follicles and many abortive hair follicles branching from the follicles and multiple immature follicles. Skin lesions were surgically excised.